MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY OF NUCLEIC-ACID CONSTITUENTS AND DINUCLEOSIDE MONOPHOSPHATE PHOTOPRODUCTS

Micellar electrokinetic capillary chromatography has been explored as an efficient and rapid method of separating the photoproducts of 2'-de oxyuridylyl-(3'-5')-thymidine (i.e., cis-syn and trans-syn cyclobutane dimers, (6-4) photoproduct, and the related Dewar valence isomer) fro m normal nucleosides and nucleotides. Three cationic surfactants, dode cyl-, tetradecyl-, and hexadecyltrimethylammonium bromide were evaluat ed and the separations compared with those obtainable with the anionic surfactant, sodium dodecylsulfate. Optimum resolution of the dinucleo side monophosphate photoproducts was obtained using tetradecyltrimethy lammonium bromide. By use of this detergent the photoproducts could al so be separated from other normal constituents in less than 6 min at - 25 kV. Dodecyl- and hexadecyltrimethylammonium bromide provided some s eparation between the various species but sodium dodecylsulfate did no t separate the UV radiation-induced products from either the parent co mpound or other dinucleoside monophosphates. Increased interaction bet ween negatively charged solutes and positively charged micelles accoun ts for the differences.