IDENTIFICATION AND REGIONAL LOCALIZATION OF A HIGHLY POLYMORPHIC DINUCLEOTIDE REPEAT D11S614 TO THE INTERVAL IN 11Q23.3 FLANKED BY RECURRENT TRANSLOCATION BREAKPOINTS

A highly informative dinucleotide repeat polymorphism has been identif ied at the D11S614 locus on chromosome 11q23. Ten different alleles ha ve been observed at this locus, and the heterozygosity frequency is ap proximately 85 %. Physical localization of this marker in a panel of s omatic cell hybrids containing chromosome 11 translocations showed tha t it maps to 11q23.3, within the interval between the recurrent t(4;11 ) leukaemia breakpoint and the t(11;22) Ewing's sarcoma breakpoint. Th is physical mapping data is consistent with the genetic mapping which indicates tight linkage to other markers in the q23.3 region including PBGD, CD3D and D11S29. Regional localization of highly informative ma rkers such as D11S614 will facilitate integration of the genetic and p hysical maps.