T. Bryndorf et al., PRENATAL-DIAGNOSIS BY FLUORESCENCE IN-SITU HYBRIDIZATION ON CHORIONICVILLUS CELLS - NONSIGNIFICANCE OF MATERNAL CELL CONTAMINATION, Fetal diagnosis and therapy, 9(2), 1994, pp. 73-76
We assessed the effect of maternal cell contamination on the sensitivi
ty of prenatal diagnosis by fluorescence in situ hybridization (FISH)
on overnight attached mesenchymal chorionic villus cells. Double targe
ts FISHs with X and Y chromosome-specific probes were performed on par
allel samples from the same pregnancies: (1) samples of assumed fetal
tissue retained during dissection, and (2) samples of suspected matern
al tissue discarded during dissection. In the karyotypically male samp
les the tissue retained during dissection contained 0-3% nuclei with t
wo X-specific signals each. In the tissue discarded from the karyotypi
cally male samples 0-50% of the nuclei had two X-specific signals each
. We conclude that given thorough dissection of chorionic villi, the s
ensitivity of prenatal diagnosis of aneuploidies by FISH on overnight
attached samples of this tissue is not critically affected by maternal
cell contamination.