STIMULATION OF GASTROINTESTINAL ANTIBODY TO SHIGA TOXIN BY OROGASTRICIMMUNIZATION IN MICE

Citation
Ma. Suckow et al., STIMULATION OF GASTROINTESTINAL ANTIBODY TO SHIGA TOXIN BY OROGASTRICIMMUNIZATION IN MICE, Immunology and cell biology, 72(1), 1994, pp. 69-74
Citations number
36
Categorie Soggetti
Cytology & Histology",Immunology
Journal title
ISSN journal
08189641
Volume
72
Issue
1
Year of publication
1994
Pages
69 - 74
Database
ISI
SICI code
0818-9641(1994)72:1<69:SOGATS>2.0.ZU;2-Z
Abstract
Shiga toxin (ST) is a protein toxin of Shigella dysenteriae type 1, a causative agent of severe diarrhoea and dysentery. In this report we d escribe the gastrointestinal secretory antibody response of mice follo wing orogastric immunization with ST. Gastrointestinal secretions were sampled by a gastrointestinal lavage technique weekly for 5 weeks aft er initial immunization. Assay of lavage samples by ELISA showed that mice vaccinated orogastrically with various doses of ST developed gast rointestinal antibody to ST in a dose-dependent manner. Serum anti-ST activity developed by 5 weeks after initial immunization. The ability of ST to act as a mucosal immune adjuvant was investigated by coadmini stration of ST and keyhole limpet haemocyanin. In contrast to cholera toxin, a potent adjuvant, ST did not demonstrate adjuvant activity. Th e mouse gastrointestinal lavage model could be useful for further anal ysis of the cellular basis of ST immunogenicity.