ROLE OF METHANOL ON THE CATALYTIC BEHAVIOR OF BETA-GLUCOSIDASE FROM FUSARIUM-OXYSPORUM

Fusarium oxysporum p-glucosidase catalyses the transglycosylation and reverse hydrolysis reaction in an aqueous solution, in the presence of a second nucleophile stronger than water, such as methanol. In transg lycosylation, the K-m values of cellobiose for the trans reaction and hydrolysis were 138.2mM and 356.5mM respectively and the competitive r ole of methanol was shown. The K, value of methanol was calculated to be 1.41M. In the reverse hydrolysis the influence of the donor concent ration, up to 90% w/v glucose, was investigated and the competitive ro le of methanol as a nucleophilic acceptor of the glycosyl moiety was a lso demonstrated.