INFLUENCE OF NONESSENTIAL AMINO-ACIDS ON APOPTOTIC AND NECROTIC DEATHOF MOUSE HYBRIDOMA CELLS IN BATCH CULTURES

Supplementing a medium with non-essential amino acids results in an in creased membrane stability during the death phase of a batch hybridoma culture (determined by Trypan Blue). The effect can only be seen when the amino acids are added at the beginning of the culture. This then leads to a different metabolism resulting in a decreased lactate conce ntration and an increased antibody secretion during the death phase. S urprisingly the addition of the amino acids has no effect on the deter mined viability (acridine orange and propidium iodine staining).