LIQUID INJECTION GAS-CHROMATOGRAPHY AND THE ALDH ASSAY OVERESTIMATE FREE ACETALDEHYDE IN COMPLEX FERMENTATION MEDIA

Authors
HOBLEY TJ PAMMENT NB
Citation
Tj. Hobley et Nb. Pamment, LIQUID INJECTION GAS-CHROMATOGRAPHY AND THE ALDH ASSAY OVERESTIMATE FREE ACETALDEHYDE IN COMPLEX FERMENTATION MEDIA, Biotechnology techniques, 11(1), 1997, pp. 39-42
Citations number
21
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
1
Year of publication
1997
Pages
39 - 42
Database
ISI
SICI code
0951-208X(1997)11:1<39:LIGATA>2.0.ZU;2-B
Abstract
Almost 75% of the free acetaldehyde added to an uninoculated culture m edium was lost upon overnight incubation due to reaction with yeast ex tract and tryptone. In the presence of these components, liquid inject ion gas chromatography and the ALDH assay substantially overestimated acetaldehyde concentrations, highlighting the importance of head space gas chromatography in obtaining reliable estimates of the free acetal dehyde concentration.