PRENATAL-DIAGNOSIS OF SICKLE-CELL-ANEMIA USING PCR AND RESTRICTION ENZYME DDE-I

Prenatal diagnosis of sickle cell anemia was carried out in four fetus es using DNA technology. Fetal chorionic villus specimen were obtained at the 10th week of pregnancy from women at risk of giving birth to c hildren with sickle cell anemia. Whole cellular DNA was obtained and t he part of the DNA presumed to have a mutation increased after PCR was performed. After the application of Dde I restriction enzyme, mini ge l electrophoresis was performed. The study of the electrophoretic patt erns of the DNA indicated that one of the four fetuses was unaffected, one was a carrier and the remaining two were affected.