POLYNUCLEAR FERTILIZATION IN VARIOUS STIMULATION PROTOCOLS IN IN-VITRO FERTILIZATION

Objective-To investigate the effect of some clinical characteristics, especially various ovarian stimulation protocols, on the occurrence of polypronuclear fertilization. Methods-One hundred forty-eight consecu tive cycles in 121 patients were treated in our IVF program and 735 oo cytes were analyzed retrospectively. Results-Multipronuclear fertiliza tion occurred in 33 of 530 fertilized oocytes, an incidence of 6.2%. N o significant difference in the incidence of polyploidy was observed b etween the three stimulation protocols (CC/hMG, hMG, GnRHa/hMG). Numbe r of polypronuclear embryos was found to be correlated with the fertil ization rate, concentration of sperm inseminated, and maturity of the oocytes. Conclusions-The maturity of the oocytes and the sperm fertili zing capacity should be examined to reduce the incidence of polypronuc lear fertilization.