DIFFERENTIAL CDNA SCREENING STRATEGIES TO IDENTIFY NOVEL STAGE-SPECIFIC PROTEINS IN THE DEVELOPING MAMMALIAN DRAIN

The molecular basis for cell lineage, migration and patterning in the developing mammalian brain is the focus of extensive investigation by neuroscientists. Many of the proteins that are uniquely expressed in s pecific stages of development remain to be isolated. In the present re view, subtractive hybridization, differential display and single cell amplification are presented as possible differential cDNA screening st rategies to identify new proteins important to neural development. The advantages and limitations of each approach are discussed and new lin es of investigation in which they can be applied effectively are sugge sted.