A. Chaudhury et al., GROWTH-BEHAVIOR OF SUSPENSION-CULTURES OF RICE AND TRANSIENT EXPRESSION OF ELECTROPORATED GENE IN INTACT-CELLS, Journal of Plant Biochemistry and Biotechnology, 3(1), 1994, pp. 9-13
Suspension cultures of Indica (Basmati 370 and Improved Sabarmati) and
japonica (Taipei 309) rice were established from scutellum-derived ca
llus obtained by culture of the mature seeds. The suspension cultures
contain groups of small and cytoplasmically rich cells with a doubling
time of 3.6-5.1 days, as measured by increase in packed cell volume.
The suspension cells of indica rice var Improved Sabarmati were employ
ed to optimize electroporation-mediated delivery of the plasmid pBI 22
1 having beta-glucuronidase gene under the control of eukaryotic expre
ssion signals. GUS activity was determined both fluorometrically and h
isotchemically. Maximum expression was obtained when cells were electr
oporated at 600 V cm(-1) with capacitance selected at 400 mu F.