SEED STORAGE PROTEIN PROFILES OF 7 INDIAN WHEAT (TRITICUM-AESTIVUM L)VARIETIES

Seed storage protein profiles, including triticin, gliadin and gluteni n, of seven Indian wheat varieties were investigated using sodium dode cyl sulfate-polyacrylamide gel eletrophoresis (SDS-PAGE). A strategy w as developed for half-seed analysis of the three seed storage protein classes. The results are presented in the form of a key for identifica tion of these varieties based on their seed protein composition. A min imum of do seeds of each variety were analysed to check for possible i ntravarietal heterogeneity, and all the varieties except HUW-12 were h omogeneous. The HUW-12 was a mixture of two 'biotypes' with respect to the low molecular weight (LMW) glutenin subunits coded by the Glu-A3 locus. The varieties were also analysed for the presence of rye chroma tin using a rye-specific repetitive DNA probe (pAW-161), and two of th e recently released varieties HUW-206 and HUW-318 were found to posses s rye chromatin. Based on the DNA dot-blot results, presence of rye se calins end the absence of chromosome 18-specific gliadins ii was concl uded that these two varieties possess 1BL-1RS wheat-rye translocation. Both of these varieties also Rave high molecular weight (HMW) gluteni n subunits 5 + 10 which may be necessary for compensating the loss of dough strength associated with the wheat-rye translocations.