S. Chand et al., SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION FROM TISSUE-CULTURES OF HYOSCYAMUS-MUTICUS L, Journal of Plant Biochemistry and Biotechnology, 3(1), 1994, pp. 53-54
Somatic embryogenesis and regeneration of plantlets was achieved in ca
llus cultures derived from cotyledonary leaf pieces of Hyoscyamus muti
cus L. on MS medium enriched with 2 mg/l 2,4-D and 0.5 mg/l BAP. For e
mbryogenesis and organogenesis varying concentrations of NAA with or w
ithout BAP were added in the medium. Organogenesis was also achieved w
hen callus was transferred to the hormone free medium.