We have applied flow cytometry to the detection of activated platelets
in patients with coronary heart disease. Paraformaldehyde-fixed plate
lets were incubated with one of the following monoclonal antibodies (M
Abs): Bx-1(anti-GP Ib), AP-2 (anti-GP IIb-IIIa complex), VH10 (anti-GM
P-140, a glycoprotein of the alpha-granule membrane), or PAC-1 (direct
ed against an activation-dependent determinant on GP IIb-IIIa complexe
s). Bound antibody was quantitated after the addition of FITC-conjugat
ed anti-immunoglobulin. This report highlights studies on 16 unstable
angina patients undergoing transluminal angioplasty. Blood samples wer
e taken at different periods before and after the angioplasty. Levels
of activated platelets were variable, remaining in the 2-4% range of c
ontrol donors for some, but increasing to 10-30% postangioplasty for o
thers (despite all patients receiving heparin and aspirin). Maximum nu
mbers of activated platelets were detected at 24 or 48 h. Nonetheless,
the amount of antibody bound to individual platelets rarely reached t
he levels seen when control platelets were stimulated with thrombin in
vitro. Results with VH10 and PAC-1 often, but not always, correlated
suggesting different pathways of platelet activation.