A PCR-BASED ASSAY FOR THE IDENTIFICATION OF THE FISH PATHOGEN RENIBACTERIUM-SALMONINARUM

Authors
LEON G MAULEN N FIGUEROA J VILLANUEVA J RODRIGUEZ C VERA MI KRAUSKOPF M
Citation
G. Leon et al., A PCR-BASED ASSAY FOR THE IDENTIFICATION OF THE FISH PATHOGEN RENIBACTERIUM-SALMONINARUM, FEMS microbiology letters, 115(2-3), 1994, pp. 131-136
Citations number
22
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
115
Issue
2-3
Year of publication
1994
Pages
131 - 136
Database
ISI
SICI code
0378-1097(1994)115:2-3<131:APAFTI>2.0.ZU;2-C
Abstract
By means of a one-step one-tube extraction from less than 1 mg of tiss ue it is possible to identify, via the polymerase chain reaction, Reni bacterium salmoninarum in salmon with bacterial kidney disease. A 149- bp DNA sequence unique to R. salmoninarum was specifically amplified a nd its nature confirmed by Southern hybridization using a non-isotopic ally labelled probe. The sensitivity of the approach allowed the detec tion of 22 R. salmoninarum cells. The procedure was successfully appli ed in the identification of the causative agent of bacterial kidney di sease in kidney tissue from infected fishes.