F. Pratvielsosa et al., EFFECT OF VARIOUS ANALOGS OF D-GLUTAMIC ACID ON THE D-GLUTAMATE-ADDING ENZYME FROM ESCHERICHIA-COLI, FEMS microbiology letters, 115(2-3), 1994, pp. 223-228
Twenty-four analogues of D-glutamic acid were tested as substrates or
inhibitors of the D-glutamate-adding enzyme from Escherichia coil. The
best substrates were, in decreasing order of specific activity, D-ery
thro-4-methylglutamic acid, D-erythro-3-methylglutamic acid, DL-homocy
steic acid, /-)-trans-1-amino-3-carboxy-cyclopentanecarboxylic acid an
d +/-)-trans-1-amino-3-carboxy-cyclohexanecarboxylic acid. Among the d
ifferent stereoisomers, only the D-erythro isomers for methylglutamic
acids, and the trans isomers for the cyclic analogs, were substrates.
Apart from the D-erythro-3- and 4-methylglutamic acids and DL-homocyst
eic acid, none of the examined compounds significantly inhibited the a
ddition of radioactive D-glutamic acid to UDP-N-acetylmuramyl-L-alanin
e.