PRETREATMENT OF CHIRAL ALPHA-AGP COLUMN WITH TRIETHYLAMINE SIGNIFICANTLY IMPROVES THE DETECTION SENSITIVITY OF AN ENANTIOMERIC LEUKOTRIENE ANTAGONIST

The peptidoleukotriene antagonist, SK&F 107310 and its optical antipod e, can be effectively separated on a chiral alpha-AGP column. Pre-trea tment of the chiral alpha-AGP column with 0.1% v/v triethylamine in th e mobile phase significantly improves the peak shape and detection sen sitivity of the undesired enantiomer. Chromatography with the modifier in the mobile phase resulted in elution of the drug substance without retention at the solvent front. Without column pre-treatment, only a broad tailing peak was obtained for enantiomer 2, making low detection of it unattainable,