DETERMINATION OF OXOLINIC ACID IN SEAWATER, MARINE SEGMENT, AND JAPANESE OYSTER (CRASSOSTREA-GIGAS) BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A reversed-phase high-performance liquid chromatographic method was de veloped to detect oxolinic acid in seawater, marine sediment, and japa nese oyster (Crassostrea gigss). Seawater was analysed after filtratio n and centrifugation. Sediment and oyster tissues were respectively an alysed after liquid-phase and solid-phase extraction. Linearity and pr ecision were checked over the concentration range 0.05-2.50 mu g/mL or mu g/g. Limits of detection and determination were respectively 0.01 and 0.04 mu g/mL or mu g/g. Recoveries of oxolinic acid were 102.1% fr om seawater, 68.1% from sediment and 88.3% from oyster. Oxolinic acid concentrations in seawater, sediment and oyster spiked at 0.50 mu g/mL or mu g/g were stable at -20 degrees C for 60 days.