AN XRF STUDY OF TRACE-ELEMENTS ACCUMULATION IN KIDNEYS OF TUMOR-BEARING MICE AFTER TREATMENT WITH CIS-DDP WITH AND WITHOUT SELENITE AND SELENOCISTAMINE
C. Shenberg et al., AN XRF STUDY OF TRACE-ELEMENTS ACCUMULATION IN KIDNEYS OF TUMOR-BEARING MICE AFTER TREATMENT WITH CIS-DDP WITH AND WITHOUT SELENITE AND SELENOCISTAMINE, Biological trace element research, 40(2), 1994, pp. 137-149
The effect of cis-DDP treatment with and without selenite and selenoci
stamine was studied on kidneys of tumor-bearing mice. The amounts of c
is-DDP, selenite, and selenocistamine injected were chosen so as to be
compatible with the treatment of humans. The animals were sacrificed
at 7, 14, and 28 d after treatment. The kidneys were removed and subje
cted to trace element analysis by a novel X-ray fluorescence (XRF) met
hod and for pathological assessment. The results show that following t
reatment with cis-DDP, K, Fe, Cu, and Zn reach a maximum level after 7
d; K, Fe, and Cu levels were significantly reduced by the addition of
selenite. The level of Zn was reduced only in groups treated with sel
enite whereas that of K and Cu was reduced also in groups treated with
selenocistamine with and without cis-DDP. The greatest increase in Pt
and Se levels was reached 1 wk after injection with cis-DDP, with and
without seleno-compounds, and in the case of Pt was partly reduced by
addition of selenite. Se returned to control values 2 wk after inject
ion, although Pt was still high in all groups 2 and 4 wk after injecti
on. The results corroborate the findings of our previous studies. The
effect of selenocistamine in cis-DDP treated mice was partly insuffici
ent. The pathological examination of the kidneys did not show any diff
erences in the effect of various additives during the study.