AN XRF STUDY OF TRACE-ELEMENTS ACCUMULATION IN KIDNEYS OF TUMOR-BEARING MICE AFTER TREATMENT WITH CIS-DDP WITH AND WITHOUT SELENITE AND SELENOCISTAMINE

The effect of cis-DDP treatment with and without selenite and selenoci stamine was studied on kidneys of tumor-bearing mice. The amounts of c is-DDP, selenite, and selenocistamine injected were chosen so as to be compatible with the treatment of humans. The animals were sacrificed at 7, 14, and 28 d after treatment. The kidneys were removed and subje cted to trace element analysis by a novel X-ray fluorescence (XRF) met hod and for pathological assessment. The results show that following t reatment with cis-DDP, K, Fe, Cu, and Zn reach a maximum level after 7 d; K, Fe, and Cu levels were significantly reduced by the addition of selenite. The level of Zn was reduced only in groups treated with sel enite whereas that of K and Cu was reduced also in groups treated with selenocistamine with and without cis-DDP. The greatest increase in Pt and Se levels was reached 1 wk after injection with cis-DDP, with and without seleno-compounds, and in the case of Pt was partly reduced by addition of selenite. Se returned to control values 2 wk after inject ion, although Pt was still high in all groups 2 and 4 wk after injecti on. The results corroborate the findings of our previous studies. The effect of selenocistamine in cis-DDP treated mice was partly insuffici ent. The pathological examination of the kidneys did not show any diff erences in the effect of various additives during the study.