INDUCTION OF HYDROLYTIC ENZYMES IN BEAN CELL AND PROTOPLAST CULTURES TREATED WITH BOTRYTIS-CINEREA MYCELIUM EXTRACT

The reaction of bean cell suspension cultures and protoplasts to treat ment with Botrytis cinerea mycelial extract has been studied. In treat ed bean cell cultures chitinase, beta-1,3-glucanase and beta-D-glucosi dase were induced. Chitinase and glucanase activities increased coordi nately during the whole examined period. Most of the induced enzymes w ere secreted into the culture medium. The presence of a new fraction o f exochitinase and of beta-1,3-glucanase in elicited bean suspensions has been shown using ion exchange chromatography. Bean protoplasts ret ain tile capability to be elicited by B. cinerea extract.