M. Garnczarska et L. Ratajczak, CHANGES IN THE ACTIVITY AND ISOZYME PATTERNS OF ALCOHOL DEHYDROGENASEIN DEVELOPING AND GERMINATING-SEEDS OF YELLOW LUPINE, Acta Physiologiae Plantarum, 15(4), 1993, pp. 257-261
A relatively high activity of alcohol dehydrogenase was observed in de
veloping seeds of yellow lupine, reaching 4 U/seed. Electrophoretic an
alysis demonstrated three molecular forms of the enzyme isolated from
embryo axes while two forms of the enzyme were present in cotyledons a
nd seed coats, corresponding to the two slowly migrating forms from em
bryo axes. Pattern of molecular forms in dry seeds and germinating see
ds was the same as that in developing seeds: cotyledons contained two
forms while embryonal seedling axes contained three forms of the enzym
e. The pattern of electrophoretic enzyme forms in cotyledons and axes
of seedlings showed no changes within 72 h of germination. However, ch
anges were observed in activity of alcohol dehydrogenase during germin
ation. In embryonic axes, th activity was increasing, reaching peak va
lues in the 24th h of imbibition followed by an abrupt decrease (after
seed coat perforation by the axis). In cotyledons the changes in acti
vity were insignificant. When coat was removed, a rapid decrease in en
zyme activity was observed in axes, associated with increase in the ac
tivity in cotyledons. In discussion, it was stressed that ADH form of
tile highest electrophoretic mobility is specific for the embryo axis.