THE INFLUENCE OF DIFFERENT TYPES OF MEDIA SUPPLEMENT ON THE MEIOTIC MATURATION OF BOVINE OOCYTES IN-VITRO

This work was designed to evaluate the influence of different types of media supplements in the maturation of preovulatory oocytes in cattle . We studied 4 different supplements: serum from estrous cattle (ECS), serum from fetal calves (FCS), amniotic fluid from cattle (bAF) and f ollicular fluid from cattle (bFF). Preovulatory bovine oocytes recover ed from ovaries of mature cows after slaughter were cultured for 24 h in TCM-199 medium containing 20% of one of these protein supplements. The percentages of oocyte maturation were significantly higher (P < 0. 001) in ECS (78%), FCS (79%) and bAF (77%) than in bFF (52%). These da ta indicate that supplementation with ECS, FCS or bFF is adequate for maturation of bovine oocytes without addition of other compounds.