Vfmh. Delima et al., EFFECT OF CELL-CYCLE SYNCHRONIZATION ON THE ACCURACY OF MURINE AND BOVINE EMBRYO SEX DETERMINATION, Theriogenology, 41(2), 1994, pp. 521-534
Different cell cycle synchronization methods were used to increase the
mitotic index and accuracy of sex determination in murine and bovine
embryos. For sexing purposes, colchicine treatment for 2, 4, 6 and 8 h
and the FdU-thymidine-colchicine combination were tested in murine em
bryos. The best results were obtained with colchicine treatment for 8
h (96.88% accuracy) and with FdU-thymidine-colchicine (97.22% accuracy
). Mitotic indexes differed significantly between the 2 treatments (21
.71% for colchicine and 32.95% for FdU-thymidine-colchicine). For sex
identification of murine and bovine demi-embryos, both treatments were
demonstrated to be equally effective (nearly 90%). The mitotic index
for the FdU-treated murine demi-embryos (19.04%) was higher than the o
ne obtained for the 8-h colchicine treatment (15.62%).