SEX DETERMINATION IN SPOTTED HYENA (CROCUTA-CROCUTA) BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF AMPLIFIED ZFX ZFY LOCI/

Authors
SCHWERIN M PITRA C
Citation
M. Schwerin et C. Pitra, SEX DETERMINATION IN SPOTTED HYENA (CROCUTA-CROCUTA) BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF AMPLIFIED ZFX ZFY LOCI/, Theriogenology, 41(2), 1994, pp. 553-559
Citations number
25
Categorie Soggetti
Veterinary Sciences
Journal title
Theriogenology → ACNP
ISSN journal
0093691X
Volume
41
Issue
2
Year of publication
1994
Pages
553 - 559
Database
ISI
SICI code
0093-691X(1994)41:2<553:SDISH(>2.0.ZU;2-A
Abstract
We describe a quick and efficient method of determining the sex of DNA samples in the hyena. By choosing primers from sequences that are con served between the human and bovine ZFY and ZFX genes, we amplified a 448 bp fragment from 1 male and 2 female hyenas. Using comparative seq uencing, single base pair polymorphisms between the amplified ZFY and ZFX were established. Restriction fragment length polymorphism (RFLP) analysis with PstI and TaqI confirmed the sequence data and yielded sp ecific banding patterns between the 2 sexes in the hyena.