NEW SITE-SPECIFIC ENDONUCLEASE AND METHYLASE FROM BACILLUS-LICHENIFORMIS-736

Authors
MATVIENKO NN KRAMAROV VM ZHELEZNAYA LA MATVIENKO NI
Citation
Nn. Matvienko et al., NEW SITE-SPECIFIC ENDONUCLEASE AND METHYLASE FROM BACILLUS-LICHENIFORMIS-736, Biochemistry, 58(8), 1993, pp. 803-814
Citations number
15
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
58
Issue
8
Year of publication
1993
Pages
803 - 814
Database
ISI
SICI code
0006-2979(1993)58:8<803:NSEAMF>2.0.ZU;2-T
Abstract
The site-specific endonuclease R Bli736I and methylase M Bli736I were isolated from strain Bacillus licheniformis 736 using blue agarose, HA -Ultragel, and heparin-Sepharose chromatography. The enzymes are free from interfering impurities. R Bli736I recognizes the [GRAPHICS] seque nce on DNA and cleaves it as indicated by the arrows, creating single- stranded 4-nucleotide 5'-protruding ends. This enzyme is an isoschizom er of Eco31I isolated from E. coli.