NEW METHOD FOR ISOLATION OF ACID-RESISTANT TRYPSIN-INHIBITOR FROM HUMAN URINE

A novel procedure for isolation of acid-resistant urine trypsin inhibi tor (UTI) is described The method is based on the use of UTI-specific monoclonal antibodies immobilized on an insoluble matrix. Utilization of Immotin (UTI immobilized on Sepharose 4B) as a precolumn allows con siderable increase in the sen,ice-life of the immune sorbent. Isolatio n of UTI by this procedure increases the yield up to 92% of original u rinary content of the inhibitor. The resulting preparation contains pr edominantly the native form of the inhibitor (-44 kD molecular weight) .