ATP-DEPENDENT STRUCTURAL-CHANGES IN CHROMATIN

Authors
TYULENEV VI KONOPLICH LA KRIVONOS AA KHRAPUNOV SN
Citation
Vi. Tyulenev et al., ATP-DEPENDENT STRUCTURAL-CHANGES IN CHROMATIN, Biochemistry, 58(8), 1993, pp. 856-861
Citations number
29
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
58
Issue
8
Year of publication
1993
Pages
856 - 861
Database
ISI
SICI code
0006-2979(1993)58:8<856:ASIC>2.0.ZU;2-7
Abstract
Incubation of nuclei and chromatin isolated from guinea pig spleen (bu t not thymus or liver) at 37 degrees C for 1 h induces proteolysis of histones H1 and H2A and generates specific products. Electrophoresis w ith sodium dodecyl sulfate reveals a decrease in contents of histones H1 and H2A, anti the appearance of proteins traveling ahead of histone H1(0) and between histones H2A and H4. Addition of to mM ATP (but nor cAMP or PPi) to the reaction mixture prevents cleavage of histones H1 and H2A and the appearance of the above described proteins. ATP prote cts the nuclear and chromatin histones from proteinases and promotes d egradation of low-molecular-weight cytosol proteins. The mechanisms of structural modifications of chromatin by ATP causing the nuclear prot eins to resist proteolysis are discussed.