STUDY OF RELATIVE HYDROPHOBICITY AND OTHER PROPERTIES OF LECTINS IN AQUEOUS 2-PHASE POLYMER SYSTEMS

The relative hydrophobicity of ten purified lectin preparations was de termined using the aqueous two-phase system Ficoll 400 (13.53 wt. %)-D extran 70 (11.59 wt. %). A linear correlation between the relative hyd rophobicity of lectins and their hemagglutinating activity towards nat ive rabbit erythrocytes was established (r(2) = 0.925). Con A revealed lower relative hydrophobicity in the presence of its low-molecular-we ight sugar inhibitors. Using cross partition, isoelectric points of ba sic lectins were calculated.