T. Kobayashi et al., THE PRESENCE OF GPI-LINKED PROTEIN(S) IN AN ARCHAEOBACTERIUM, SULFOLOBUS-ACIDOCALDARIUS, CLOSELY-RELATED TO EUKARYOTES, Biochimica et biophysica acta (G). General subjects, 1334(1), 1997, pp. 1-4
GPI-anchored proteins are distributed ubiquitously in eukaryotes, but
not in procaryotes. By methabolic-labeling of Sulfolobus acidocaldariu
s cells, C-14-radiolabeled precursors of GPI and caldarchaetidylinosit
ol were incorporated into 120, 143 and 185 kDa proteins. The 185 kDa p
rotein was specifically solubilized by bacterial phosphatidylinositol-
specific phospholipase C. Therefore, Sulfolobus proved to contain at l
east one GPI-anchored proteins.