FC-GAMMA-RII CROSS-LINKING INHIBITS ANTI-IG-INDUCED EGR-1 AND EGR-2 EXPRESSION IN BCL(1)

Citation
Ar. Gottschalk et al., FC-GAMMA-RII CROSS-LINKING INHIBITS ANTI-IG-INDUCED EGR-1 AND EGR-2 EXPRESSION IN BCL(1), The Journal of immunology, 152(5), 1994, pp. 2115-2122
Citations number
52
Categorie Soggetti
Immunology
Journal title
The Journal of immunology
ISSN journal
00221767 → ACNP
Volume
152
Issue
5
Year of publication
1994
Pages
2115 - 2122
Database
ISI
SICI code
0022-1767(1994)152:5<2115:FCIAEA>2.0.ZU;2-4
Abstract
Activation of B cells through their Ag receptor is known to be negativ ely regulated by Fc gamma RII engagement. To explore the molecular and biochemical mechanisms of Fc gamma RII-mediated inhibition, we invest igated the effect of Fc gamma RII engagement on the expression of two immediate-early genes, egr-1 and egr-2, and tyrosine phosphorylation e vents following the activation of the murine B cell line, BCL(1). Egr- 1 and egr-2 were expressed in BCL(1) after slg cross-linking. The indu ction of egr-1 and egr-2 expression was prevented when the Fc gamma RI I was co-cross-linked with sIg in BCL(1), but not in WEHI-231. The inh ibitory effects of Fc gamma RII engagement on egr-1 and egr-2 expressi on occurred when the Fc gamma RII was cross-linked with either sIgM or sIgD. Treatment with cyclosporin A prevented the expression of egr-2 induced by sig cross-linking, but did not inhibit egr-1 expression. In addition, cyclosporin A did not prevent the negative-regulatory effec ts of Fc gamma RII engagement on egr-1 expression, suggesting that the Fc gamma RII works upstream from the site of action of cyclosporin A, To investigate activation signals more proximal to the plasma membran e, we compared tyrosine phosphorylation patterns of several effector m olecules known to play a role in B cell activation. Cross-linking of s Ig induced tyrosine phosphorylation of the p62 CAP-associated protein. The p62 protein became hyperphosphorylated in response to co-cross-li nking of sIg with Fc gamma RII. Our results identify egr-1 and egr-2 a s targets of Fc gamma RII-mediated inhibition of anti-Ig-induced B cel l activation. In addition, they show that negative regulation by Fc ga mma RII is effective in both cyclosporin A-sensitive and insensitive p athways. Further, we suggest a possible Fc gamma RII signaling pathway leading to the inhibition of egr-1 and egr-2 expression.