L. Rahalison et al., ANTIFUNGAL TESTS IN PHYTOCHEMICAL INVESTIGATIONS - COMPARISON OF BIOAUTOGRAPHIC METHODS USING PHYTOPATHOGENIC AND HUMAN PATHOGENIC FUNGI, Planta medica, 60(1), 1994, pp. 41-44
The detection limits in two bioautographic assays have been determined
for a series of antifungal compounds, including clinically used antim
ycotics, fungicidal agrochemicals, and various classes of secondary pl
ant metabolites. Target organisms were the filamentous fungus Cladospo
rium cucumerinum and the yeast Candida albicans. For clinical agents a
nd agrochemicals, the detection limits in the two assays reflected to
a certain extent their known spectrum of activity. Most of the plant-d
erived compounds tested showed a positive response in both assays, but
with detection limits varying by a factor up to tenfold. For screenin
g purposes, it is thus advisable to use both tests, as some activities
would otherwise go undetected. The MIC values of these substances wer
e determined in order to verify a possible correlation with the detect
ion limit in the bioautographic assays.