DOMINANT-NEGATIVE INHIBITION OF TUMORIGENESIS IN-VIVO BY HUMAN INSULIN-LIKE GROWTH-FACTOR-I RECEPTOR MUTANT

Although insulin-like growth factor I(IGF-I) is a mitogenic growth fac tor, its role in tumorigenesis is unclear. We therefore transfected wi ld-type and truncated beta-subunit mutant ((STOP)-S-952) human IGF-I r eceptor cDNAs into Rat-1 fibroblasts. Rat-1 transfectants expressed 2. 5- to 7-fold increased IGF-I receptor mass, while the K-d for IGF-I bi nding was unchanged. The Rat-1 cells transfected with wild-type recept or cDNA responded to in vitro IGF-I treatment by increased proliferati on and DNA synthesis. Cells overexpressing wild-type receptors were al so transformed as evidenced by ligand-dependent colony proliferation i n soft agar. After injection into athymic nude mice, al! wild-type tra nsfectants formed solid sarcomas within 3 weeks, and ex vivo tumor cel l assays confirmed continued overexpression of human IGF-I receptors. In contrast, both DNA synthesis and proliferation of (STOP)-S-952-tran sfected cells were attenuated below that of untransfected cells. (STOP )-S-952 cells were nonresponsive to IGF-I in vitro and were unable to sustain anchorage-independent growth. No tumors were induced for up to 8 weeks after injection of (STOP)-S-952 transfectants into athymic mi ce, despite the presence of demonstrable endogenous IGF-I recep tors o n the (STOP)-S-952-transfected cells. Therefore, (STOP)-S-952 behaves as a dominant negative inhibitor of endogenous IGF-I receptor function , probably by assembling nonfunctional hybrid rat/mutant human recepto r tetramers.