ANTIGENIC COMPOSITION OF PASTEURELLA-HAEMOLYTICA SEROTYPE-1 SUPERNATANTS FROM SUPPLEMENTED AND NONSUPPLEMENTED MEDIA

Supernatant preparations containing antigens produced by Pasteurella h aemolytica serotype 1 grown in nonsupplemented RPMI 1640 medium and 3 grown in supplemented RPMI 1640 media were evaluated, Antigens were de tected by immunoblotting each supernatant preparation with sera from 2 0 cattle with various degrees of resistance to experimentally induced pneumonic pasteurellosis. Antigen-antibody bands at 49 and 30 kd were correlated with resistance in all 4 media. A 66-kd antigen antibody ba nd was correlated with resistance in 2 media, and antigen-antibody ban ds at 100 and 16 kd were correlated with resistance in 1 medium each. These results indicated that the number and relative amount of resista nce-associated antigens in P haemolytica supernatants can be optimized on the basis of type of growth medium used.