PHOTOTOXICOLOGY .1. LIGHT-ENHANCED TOXICITY OF TNT AND SOME RELATED-COMPOUNDS TO DAPHNIA-MAGNA AND LYTECHINUS-VARIAGATUS EMBRYOS

Many environmental pollutants interact with solar near-ultraviolet (nu v) light in a manner which greatly increases their toxic effects. The phenomenon of light-mediated toxicity (phototoxicity) is only now beco ming generally recognized to any significant degree. Manufacture of, a nd loading munitions with, the explosive 2,4,6-trinitrotoluene (TNT) i n past decades caused contamination of soils and sediments at levels e xceeding 1000 ppm and of waters at levels near saturation (100 ppm). M anufacture of TNT produces numerous nitrated by-products, and most of these compounds, including TNT, can be metabolized by many species, in cluding bacteria, fungi, plants, and mammals. This study investigated the phototoxicity of TNT, and 2,3-, 2,4-, 2,6-, and 3,4-dinitrotoluene (DNT) and -diaminotoluene (DAT), and the major metabolites 2-amino-4, 6-dinitrotoruene (2A) and 4-amino-2,6-dinitrotoluene (4A), to Daphnia magna (acute toxicity) and Lytechinus variagatus (sea urchin) embryos (subacute, developmental toxicity). Most of the compounds were weakly toxic or nontoxic in the dark. All were phototoxic to sea urchins. In D. magna, 2,3- and 3,4-DNT/DAT and 4A were not toxic but were phototox ic, and 2A was toxic and phototoxic; the other isomers were not toxic or phototoxic to this species. (C) 1994 Academic Press, Inc.