Dc. Rule, STARVATION-REALIMENTATION OVERSHOOT IN GLYCEROPHOSPHATE ACYLTRANSFERASE IN ADIPOSE-TISSUE AND LIVER OF RATS IS INFLUENCED BY TYPE OF DIETARY-FAT, Journal of nutritional biochemistry, 5(3), 1994, pp. 161-166
The purpose of this study was to determine effects of dietary fat sour
ce on changes in glycerophosphate acyltransferase that occur with star
vation-realimentation. Forty male Sprague-Dawley rats were randomly as
signed to dietary treatments at 209 +/- 3 g of body weight. Ten rats e
ach were assigned to a 65 g/100 g sucrose diet that contained 5 g/100
g fat from the following sources: stearate (42% stearate, 41% oleate);
oleate (high-oleate safflower oil); palm oil; or beef tallow. Five ra
ts received free access to their diet for 4 days and five rats were st
arved for 2 days and then fed for 2 days. Rats were then killed and li
ver and epididymal adipose tissue dissected and homogenized for glycer
ophosphate acyltransferase assay. The starvation-realimentation regime
n on the stearate diet stimulated adipose tissue glycerophosphate acyl
transferase above non-starved controls. Realimentation on high-oleate
and high-palmitate diets stimulated liver glycerophosphate acyltransfe
rase most as well as produced a starvation-realimentation overshoot in
steady-state glycerolipid production. Results indicate that ad libitu
m consumption of diets diets containing high proportions of stearate a
fter a period of starvation could overshoot liver glycerophosphate acy
ltransferase to a lesser extent than if the diet contained low proport
ions of this fatty acid. Diet fatty acid composition had less impact o
n adipose tissue than liver glycerophosphate acyltransferase activity
in rats subjected to starvation-realimentation.