ANALYSIS OF POPPY STRAW AND POPPY STRAW CONCENTRATE BY REVERSED-PHASEHIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
GOMEZSERRANILLOS P CARRETERO E VILLAR A
Citation
P. Gomezserranillos et al., ANALYSIS OF POPPY STRAW AND POPPY STRAW CONCENTRATE BY REVERSED-PHASEHIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Phytochemical analysis, 5(1), 1994, pp. 15-18
Citations number
35
Categorie Soggetti
Biology,"Chemistry Analytical","Plant Sciences
Journal title
Phytochemical analysis → ACNP
ISSN journal
09580344
Volume
5
Issue
1
Year of publication
1994
Pages
15 - 18
Database
ISI
SICI code
0958-0344(1994)5:1<15:AOPSAP>2.0.ZU;2-5
Abstract
A method of reversed-phase high-performance liquid chromatography usin g an elution gradient has been developed to analyse morphine, codeine, thebaine, and oripavine - this last-mentioned substance for the first time - in samples of poppy straw and poppy straw concentrate. Gradien t elution of the octadecylsilane column was performed: the gradient wa s from 10 to 30% of solvent A (methanol) in solvent B (containing 67% of 0.5% aqueous ammonium acetate solution containing diethylamine, and 33% of methanol) over a 25 min period. The four alkaloids were identi fied and quantified with the aid of an internal standard.