PURIFICATION OF XYLANASE FROM TRICHODERMA-VIRIDE BY PRECIPITATION WITH AN ANIONIC POLYMER EUDRAGIT S-100

Authors
GUPTA MN DONG GQ KAUL R MATTIASSON B
Citation
Mn. Gupta et al., PURIFICATION OF XYLANASE FROM TRICHODERMA-VIRIDE BY PRECIPITATION WITH AN ANIONIC POLYMER EUDRAGIT S-100, Biotechnology techniques, 8(2), 1994, pp. 117-122
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
2
Year of publication
1994
Pages
117 - 122
Database
ISI
SICI code
0951-208X(1994)8:2<117:POXFTB>2.0.ZU;2-2
Abstract
Endo-xylanase from T. viride has been purified 4.2 fold by precipitati on with a commercially available enteric polymer Eudragit S-100. Elect rophoretic analysis also indicated removal of contaminant proteins. Th e enzyme could be recovered in more than 89% yield. The binding of the enzyme to the polymer was predominantly by electrostatic interaction.