INCREASING GLUCOSE DETERMINATION RANGE BY FLOW-INJECTION ANALYSIS (FIA) USING GLUCOSE-OXIDASE IMMOBILIZED ON POLYANILINE

Authors
LEITE V DASILVA VL AZEVEDO WM MELO EHM FILHO JLL
Citation
V. Leite et al., INCREASING GLUCOSE DETERMINATION RANGE BY FLOW-INJECTION ANALYSIS (FIA) USING GLUCOSE-OXIDASE IMMOBILIZED ON POLYANILINE, Biotechnology techniques, 8(2), 1994, pp. 133-136
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
2
Year of publication
1994
Pages
133 - 136
Database
ISI
SICI code
0951-208X(1994)8:2<133:IGDRBF>2.0.ZU;2-L
Abstract
A Flow Injection Analysis (FIA) for glucose using glucose oxidase (E.C .1.1.3.4) was developed. The enzyme was immobilised on polyaniline che mically synthetized on internal surface of silicon tube (2.0 mm of dia meter and length from 1 to 5 meters), using glutaraldehyde as a bifunc tional agent. The system was able to measure glucose at levels from 5 to 500mM with a response time of 2min using 250mul of sample. The syst em has been operated satisfactorily for one month with more than 300 a ssays with loss of 60% of activity.