THE COMPLETE SEQUENCE OF AN 18,002-BP SEGMENT OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-XI CONTAINS THE HBS1, MRP-L20 AND PRP16 GENES, AND 6 NEW OPEN READING FRAMES

We report the sequence of an 18,002 bp DNA fragment from the right arm of Saccharomyces cerevisiae chromosome XI. This segment contains nine complete open reading frames (ORFs), YKR401 to YKR409, and part of an other ORF, YKR400, covering altogether 87.2% of the entire sequence. O ne of them, YKR400, encodes an NAD-dependent 5,10-methylene-tetrahydro folate dehydrogenase. YKR404, YKR405 and YKR406 correspond to the prev iously characterized HBS1, MRP-L20 and PRP16 genes, coding for a trans lation elongation factor, a mitochondrial ribosomal protein and an ATP -binding protein, respectively. The putative product of YKR407 contain s the zinc-binding region signature of neutral zinc metallopeptidases. The five other ORFs do not show significant homology to any known pro tein. The sequence data reported here have been assigned EMBL accessio n number Z27116.