Ms. Hetherington et al., A QUANTITATIVE-ANALYSIS OF THE BIOGENIC-AMINES IN THE CENTRAL GANGLIAOF THE POND SNAIL, LYMNAEA-STAGNALIS (L), Comparative biochemistry and physiology. Part C, Pharmacology toxicology & endocrinology, 107(1), 1994, pp. 83-93
Biogenic amines in the central ganglia of Lymnaea stagnalis have been
identified, quantified and localised using the techniques of high perf
ormance liquid chromatography (HPLC), radioenzymatic assay (REA) and f
luorescence microscopy. HPLC indicated the presence of dopamine (DA),
(5-hydroxytryptamine (5-HT), noradrenaline (NA) and 3,4-dihydroxypheny
lacetic acid (DOPAC) in Lymnaea central nervous tissue. REA confirmed
the presence of DA and NA in this tissue and, in addition, indicated t
hat some adrenaline (Adr) may be present. Quantitatively, DA and 5-HT
were the most significant amines detected, with the pedal ganglia cont
aining the highest concentrations of both. High degrees of variation i
n DA and 5-HT concentrations were observed, both between animals from
within the same sample group and between the mean values determined fr
om separate groups. Whilst there was no obvious explanation for the di
fferences between the sample group means, some evidence accrued to sug
gest that underlying seasonal variability may have been a contributory
factor.