SEROTONIN AND BETA-PHENYLETHYLAMINE DEAMINATION BY SEMICARBAZIDE-SENSITIVE AMINE OXIDASE FROM CALLISTA CHIONE HEPATOPANCREAS

Authors
NICOTRA A SANTANGELO G SENATORI O
Citation
A. Nicotra et al., SEROTONIN AND BETA-PHENYLETHYLAMINE DEAMINATION BY SEMICARBAZIDE-SENSITIVE AMINE OXIDASE FROM CALLISTA CHIONE HEPATOPANCREAS, Comparative biochemistry and physiology. Part C, Pharmacology toxicology & endocrinology, 107(1), 1994, pp. 143-147
Citations number
30
Categorie Soggetti
Biology,"Pharmacology & Pharmacy",Toxicology
Journal title
Comparative biochemistry and physiology. Part C, Pharmacology toxicology & endocrinology → ACNP
ISSN journal
13678280
Volume
107
Issue
1
Year of publication
1994
Pages
143 - 147
Database
ISI
SICI code
1367-8280(1994)107:1<143:SABDBS>2.0.ZU;2-4
Abstract
Amine oxidase activity against 5-HT and PEA has been studied in hepato pancreas homogenates of Callista chione (Mollusca, Bivalvia). Specific activity values were 3 pmoles/mg protein/min and 0.05 nmoles/mg prote in/min with 5-HT and PEA, respectively. Values of activities measured with the two substrates were differently affected by variations of pH (6.5-8) and temperature (17 degrees C-67 degrees C). Monoamine oxidase inhibitors such as clorgyline and deprenyl used at a concentration of 10(-4) M did not significantly affect 5-HT and PEA deamination. Semic arbazide 10(-4) M completely inhibited deamination of both substrates indicating that, at least under the experimental conditions adopted, 5 -HT and PEA were deaminated by a semicarbazide-sensitive amine oxidase .