M. Senkal et al., MITOXANTRONE-INDUCED DNA STRAND BREAKS IN CELL-CULTURES OF MALIGNANT HUMAN ASTROCYTOMA AND GLIOBLASTOMA TUMORS, Journal of neuro-oncology, 32(3), 1997, pp. 203-208
In this study mitoxantrone (Mtx) induced DNA strand breaks were measur
ed with the alkaline elution technique in short term cell cultures der
ived from human gliomas. Glioblastomas or astrocytomas from 5 patients
who underwent intracranial surgery were cultured and incubated 1 h wi
th different concentrations of Mtx (0, 0.01, 0.1 and 1.0 mu g/ml). The
alkaline elution method was modified to measure DNA lesions in human
gliomas. Mtx induced DNA strand breaks in a dose dependent manner in a
ll cell cultures tested. There was a linear increase of DNA strand bre
ak frequency induced by Mtx between 0.01-1.0 mu g/ml. Concerning these
in vitro data, Mtx might be potentially useful for the treatment of p
atients with malignant brain tumors.