EFFECT OF IRRADIATION ON THE DETECTION OF BACTERIAL-DNA IN CONTAMINATED FOOD SAMPLES BY DNA HYBRIDIZATION

Authors
ROWE TF TOWNER KJ
Citation
Tf. Rowe et Kj. Towner, EFFECT OF IRRADIATION ON THE DETECTION OF BACTERIAL-DNA IN CONTAMINATED FOOD SAMPLES BY DNA HYBRIDIZATION, Letters in applied microbiology, 18(3), 1994, pp. 171-173
Citations number
9
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
18
Issue
3
Year of publication
1994
Pages
171 - 173
Database
ISI
SICI code
0266-8254(1994)18:3<171:EOIOTD>2.0.ZU;2-U
Abstract
A membrane-based DNA hybridization technique was used in a model syste m to examine the effect of irradiation treatment on the detection of b acterial contamination in foodstuffs. Although hybridization signals w ere reduced compared with otherwise identical unirradiated food sample s, artificial contamination levels in excess of 10(5) cfu per test cou ld be distinguished in 12 of the 13 foods examined following the irrad iation process. In no case were viable bacteria detected following irr adiation treatment.