CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PLASTOCYANIN FROM SILENE EXPRESSED IN ESCHERICHIA-COLI

Authors
LI CM INOUE T GOTOWDA M HAMADA K NISHIO N HIBINO T TAKABE T KAI Y
Citation
Cm. Li et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PLASTOCYANIN FROM SILENE EXPRESSED IN ESCHERICHIA-COLI, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 129-130
Citations number
18
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
1
Pages
129 - 130
Database
ISI
SICI code
0907-4449(1997)53:<129:CAPSOP>2.0.ZU;2-9
Abstract
Plastocyanin from Silene (SilPc) expressed in E. coli has been crystal lized in a form suitable for X-ray diffraction analysis by a macroseed ing method using ammonium sulfate as a precipitant in acetate buffer ( pH=5.5). These crystals belong to the trigonal space group P3(1)21 or P3(2)21 with lattice parameters a = b = 76.6, c = 65.5 Angstrom, indic ating an asymmetric unit containing two plastocyanin molecules. The cr ystals diffracted to at least 2.0 Angstrom.