HUMAN SERUM-ALBUMIN INTERACTIONS WITH BIL IRUBIN IN A SOLUTION AND A SUSPENSION CONTAINING MEMBRANOUS PARTICLE BILIARY PIGMENT

Spectrophotometrically measured in homogenous aqueous medium associati on constants for the formation of bilirubin - human serum albumin comp lex did not correlate with the intensity of the same complex formation as a result of bilidiene release from nerve cell plasma membrane part icles containing biliary pigment in aqueous medium during changed hydr ogen parameter and ionic strength of the medium (pH22-degrees 6.8, 7.2 , or 7.5 and NaCl concentration in the medium 0.135, 0.150, or 0.165 M ), such changes being compatible with normal mammalian cellular and ti ssue vital activity, the rest conditions being compatible. Study of th e time course of the said complex formation in membranous particle sus pension seems to be more adequate and therefore more reliable for orga nic anions (e.g. bilirubin) transport related assessment of detoxifyin g activity of infused human serum albumin preparations than measuremen t of the said association constants in a homogenous aqueous medium.