INTERGENERIC CONJUGATION ESCHERICHIA-COLI STREPTOMYCES AS A MEANS FORTHE TRANSFER OF CONJUGATIVE PLASMIDS INTO PRODUCERS OF ANTIBIOTICS CHLORTETRACYCLINE AND BIALAPHOS

Authors
TABAKOV VY VOEYKOVA TA TOKMAKOVA IL BOLOTIN AP VAVILOVA EY LOMOVSKAYA ND
Citation
Vy. Tabakov et al., INTERGENERIC CONJUGATION ESCHERICHIA-COLI STREPTOMYCES AS A MEANS FORTHE TRANSFER OF CONJUGATIVE PLASMIDS INTO PRODUCERS OF ANTIBIOTICS CHLORTETRACYCLINE AND BIALAPHOS, Genetika, 30(1), 1994, pp. 57-61
Citations number
14
Categorie Soggetti
Genetics & Heredity
Journal title
Genetika → ACNP
ISSN journal
00166758
Volume
30
Issue
1
Year of publication
1994
Pages
57 - 61
Database
ISI
SICI code
0016-6758(1994)30:1<57:ICESAA>2.0.ZU;2-V
Abstract
A system for introduction of plasmids into industrial producers of ant ibiotics chlortetracycline and bialaphos using intergeneric conjugatio n of Escherichia coli and Streptomyces was developed. Low level stabil ity of inheritance of autonomously replicating DNA in recipient strain s was shown. Site-specific integration of the conjugative-integrative vector pT01 provided stable plasmid maintenance within the chromosomes of Streptomyces aureofaciens and S. hygroscopicus. Phenomenon of dist urbance in differentiation and antibiotic production, resulting from p T01 integration into S. hygroscopicus chromosome, was discovered.