RAPID KARYOTYPING OF MAMMALIAN-CELLS

The use of ''pipette'' method ensures rapid preparation of standardize d whole metaphase spreads. Experiments with human, murine, Chinese ham ster, American mink, green African monkey, dog, and vole cells demonst rated that G-banded whole metaphase spreads can be obtained in less th an two hours after the beginning of work with cell or tissue culture. Due to that, it became possible to start karyotyping of animal tissue explants, as well as fetal cells present in human amniotic fluid, on d ay 3 to 4 after their receiving.