Periodate oxided starch (oxystarch - a starch derivative) is shown to
be a good supporter in a direct immobilization process for enzymes. Im
mobile conditions and properties of immobilized enzyme are discussed.
The effects of various factors, such as pH value, aldehydic groups con
tent in oxystarch, ratio of enzyme to supporter, reaction time and tem
perature, on urease immobilization were studied, respectively, and on
these results an optimum immobile condition could be decided. In addit
ion, some physical and chemical properties of immobilized enzymes were
examined. The stability of immobilzate is superior to that of the ori
ginal enzyme, and its Km value is higher than that of the original one
.