M. Ishibe et al., LOW CONTENT OF ESTROGEN-RECEPTORS IN HUMAN GIANT-CELL TUMORS OF BONE, Archives of orthopaedic and trauma surgery, 113(2), 1994, pp. 106-109
We tried to identify estrogen receptors (ERs) in human giant cell tumo
rs of bone. Samples from eight patients were used for biochemical and
immunohistochemical studies using [I-125] 17beta-estradiol and monoclo
nal antibody against the human ER. Estradiol binding sites were detect
ed in seven cases out of eight. The dissociation constant of the highe
r affinity binding site was 0.5 nM. Nonlabeled 17beta-estradiol and sy
nthetic estrogen, diethylstilbestrol, inhibited the binding of labeled
17beta-estradiol to the tumor cytosol, while dexamethasone did not in
hibit the binding. Using a monoclonal antibody, Western blotting ident
ified bands of M(r) 68000 and 59000. No ERs were observed in any case
examined immunohistochemically. These results suggest that human giant
cell tumors of bone have a low level of ERs that cannot be detected i
mmunohistochemically.