BIOCHEMICAL AND CHEMOTAXONOMIC CHARACTERIZATION OF PSEUDOMONAS-STUTZERI GENOMOVARS

Citation
Ra. Rossellomora et al., BIOCHEMICAL AND CHEMOTAXONOMIC CHARACTERIZATION OF PSEUDOMONAS-STUTZERI GENOMOVARS, Journal of Applied Bacteriology, 76(3), 1994, pp. 226-233
Citations number
24
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00218847
Volume
76
Issue
3
Year of publication
1994
Pages
226 - 233
Database
ISI
SICI code
0021-8847(1994)76:3<226:BACCOP>2.0.ZU;2-O
Abstract
A total of 48 strains representing the seven Pseudomonas stutzeri geno movars (DNA/DNA homology groups) were studied for cellular fatty acid composition, physiological characteristics and protein profiles. All s trains were found to be homogeneous with respect to their fatty acid p atterns. Numerical analysis of physiological properties demonstrated a considerable phenotypic heterogeneity within the genomovars. Characte rization of the individual genomic groups on the basis of biochemical tests was not possible. In a numerical study of cellular protein patte rns, two main clusters were obtained, one representing genomovars 1, 6 and 7 characterized by a high G + C content (mean value > 64 mol%), t he other representing genomovars 2, 3, 4 and 5 with a low G + G conten t (< 64 mol%). The standardized cellular protein patterns have potenti al for differentiation of the genomic groupings within the species Ps. stutzeri as currently circumscribed.